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Raihan A S M A

Raihan A S M A

Bangabandhu Sheikh Mujib Medical University, Bangladesh

Title: Association of nontuberculous mycobacteria (NTM) with Crohn's disease (CD)

Biography

Biography: Raihan A S M A

Abstract

Introduction: The most widely held hypothesis on the pathogenesis of IBD (Inflammatory Bowel Disease) is that overly aggressive acquired (T cell) immune responses to a subset of commensal enteric bacteria develop in genetically susceptible hosts, & environmental factors precipitate the onset or reactivation of disease. But it is almost universally accepted that a host genetic predisposition is critical for development of CD. Among bacteria Mycobacterium avium subsp paratuberculosis (MAP) has shown high prevalence although not uniformly. This large variation may be due to differences in DNA extraction techniques or to geographical variations in the prevalence and modes of transmission of MAP.

Aim & Objectives: This prospective study aimed to see the association of NTM with Crohn’s disease.

Methods: Patients of CD were included in the study as case. Patients of IBS (Irritable Bowel Syndrome) or suspected colorectal malignancy who were found to be normal at colonoscopy and colonic biopsy was normal, were included in the study as control. Two bites of biopsy from the lesion in CD patients were taken for NTM by PCR. In control group two bites were taken for histopathology and two bites were taken for NTM PCR from left side of the colon. We used ITS primer for NTM and IS 900 primer for Mycobacterium avium sub species paratuberculososis (MAP) in PCR. We extracted the DNA from tissue for PCR using QIAamp DNA mini kit.

Results: We studied 34 CD patients and 34 controls. Out of 34 cases 28 were found to be positive for nontuberculous mycobacteria (NTM) and 6 were negative. Out of 34 controls only 8 were found to be positive for nontuberculous mycobacteria (NTM) and 26 were negative. Odds ratio 15.17 (95 % CI 4.07-60.75) and P value was significant (0.001). Among them we couldn’t detect MAP neither in cases nor in controls.
 

Conclusions: From this study it appears that NTM other than MAP may have association with Crohn's disease in Bangladesh.