Efren Mireles Sandoval
Medical Doctor, México
Title: Use of extracellular matrix in intestinal anastomosis and burst testing in wistar model University India
Biography
Biography: Efren Mireles Sandoval
Abstract
Introduction:
Intestinal Anastomosis is a surgical procedure that must be conducted correctly, with an optimal cicatrization, without morbidity, a rapid return to intestinal function, in order to prevent common clinical anastomosis leakage, dehiscence and adherence. Extracellular matrix is a tridimensional network that regenerates, protects and nurtures tissue. The objective of this study was to evaluate and compare the bursting strength of the intestinal anastomosis using the extracellular matrix.
Material and Methods:
In 12 Wistar rats, females of 12 to 16 weeks of age with a weight of 200-400 g, were anesthetized, posteriorly a trichotomy was realized and disinfection with iodine. To the control group an entero-entero anastomosis end to end was made. To the experimental group the same procedure and extracellular matrix was infiltrated. At 7 days euthanasia was realized in a CO2 chamber, afterwards the burst strength testing was done. A T student test was made with a confidence interval of 95% and a level of significance of 0.05% in SPSS.
Results:
In the control group the mean bursting pressure was 251.67 mmHg with a standard deviation of 40.702, in the experimental group the mean was 310 mmHg with a standard deviation of 10.954. with a statistical significance between both groups.
Conclusions:
The use of extracellular matrix as adjuvant in the intestinal anastomosis improves significantly the resistance in the burst strength test. Which could be a good option to use in surgical anastomosis and other future studies.